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Invitrogen™ Topoisomerase I

Catalog No. 38042024
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500 U
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Catalog No. Quantity
38042024 500 U
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Catalog No. 38042024 Supplier Invitrogen™ Supplier No. 38042024
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Description

Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds

  • Topoisomerase I is active in the presence of EDTA
  • Source: Purified from calf thymus
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA
  • Unit Definition: One unit catalyzes the conversion of 0.5μg of superhelical ΦX174 RF DNA to a relaxed state in 30 min. at 37°C
  • Unit Reaction Conditions: 50mM Tris-HCl (pH 7.5), 50mM KCl, 10mM MgCl2 , 0.1mM EDTA, 0.5mM DTT, 30 μg/mL BSA, 0.5μg ΦX174 RF DNA, and enzyme in 50μL for 30 min. at 37°C

Relaxing positively and negatively supercoiled DNA, Producing DNA topoisomers, Cloning, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Wet ice

Specifications

Content And Storage Store in freezer (-5 to -30°C).
Shipping Condition Wet Ice
Enzyme Topoisomerase
Quantity 500 U
Product Type Topoisomerase I

For Research Use Only. Not for use in diagnostic procedures.