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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

$716.00 - $2145.00

Specifications

Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
Cloning Method TOPO™-TA
Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
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Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

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Description
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Specifications

Specifications

TOP10
TOPO™-TA
Kit
Chemically Competent
Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Cloning Kit
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For Research Use Only. Not for use in diagnostic procedures.