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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCRII-TOPO Vector and One Shot™ TOP10 Electrocomp™ E. coli DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

$826.00 - $1,416.00

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid.


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
K466001 Invitrogen™
LSK466001
25 reactions Each for $826.00
K466040 Invitrogen™
LSK466040
50 reactions Each for $1,416.00
Specifications

Description

Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning