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  6. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Catalog No. p-4924883
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No. of Reactions:
20 Reactions
40 Reactions
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Catalog No. No. of Reactions
K202020 20 Reactions
K202040 40 Reactions
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Catalog No. K202020 Supplier Invitrogen™ Supplier No. K202020
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Description

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of the PCR product
  • Streamlined: does not require the use of PCR primers that contain restriction sites

  • Provides 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of insert
  • M13 forward and reverse primer sites for sequencing
  • How TA Cloning Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning

Specifications

Bacterial or Yeast Strain Not Included
Cloning Method TA Cloning
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 20 Reactions
Product Line TA Cloning
Product Type Cloning Kit
Promoter T7
Quantity 20 reactions
Vector pCR2.1
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For Research Use Only. Not for use in diagnostic procedures.