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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli 
Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector
Supplier: Invitrogen™ K203040
Description
The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 promoter for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- How TA Cloning™ Works
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
- Llinearized vector supplied in this kit has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
- Kit Configurations
- TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot™ TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes
Cloning, PCR Cloning
Specifications
| TOP10F Ì | |
| TA Cloning | |
| Kit | |
| 40 Reactions | |
| Cloning Kit | |
| 40 reactions |
| Chemically Competent E. coli | |
| TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
|
| PCR Cloning | |
| One Shot™ | |
| T7 | |
| pCR2.1 |
For Research Use Only. Not for use in diagnostic procedures.