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Invitrogen™ TA Cloning™ Kit, Dual Promoterwith pCR™ 2.1 Vector without Competent Cells DFS Item

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

$654.00 - $1,082.00

Specifications

Includes Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.
Storage Requirements Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



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Catalog Number Mfr. No. Bacterial Content Vector Quantity Price Quantity  
Catalog Number Mfr. No. Bacterial Content Vector Quantity Price Quantity  
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Specifications

Description

The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and Sp6 promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing
  • Cloning Method: Restriction Enzyme/MCS

  • How TA Cloning Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector
  • Kit Configurations
  • TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning

Description & Specifications

Specifications

Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.
Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.