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Invitrogen™ SuperScript™ Direct cDNA Labeling Module, no purification

Catalog No. L101503
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L101503 30 Reactions
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Catalog No. L101503 Supplier Invitrogen™ Supplier No. L101503
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Description

Includes

The SuperScript™ Direct cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, Anchored Oligo(dT), Random Primers, control RNA, 5X First-Strand Buffer, 0.1M DTT, 10mM dNTP Mix, and DEPC-treated water. The SuperScript™ Direct cDNA Labeling System includes all components listed for the SuperScript™ cDNA Labeling Module plus purification columns, loading buffer, wash buffer, and amber collection tubes.

Provides the core kit reagents for the SuperScript Indirect cDNA Labeling System, but no purification capacity

The SuperScript Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. Modules do not include all the components. The SuperScript Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample. In this method, anchored oligo(dT) anneals to the mRNA template. SuperScript III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.

  • Provides the flexibility to use fluorescent nucleotides
  • High cDNA yields without an additional enzyme “spike”
  • Strong signal intensities
  • A fast and simple protocol that minimizes “hands on” time and provides superior reproducibility

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis

Specifications

Product Type cDNA Labeling Module
Detection Method Fluorescence
Content And Storage The SuperScript™ Direct cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, Anchored Oligo(dT), Random Primers, control RNA, 5X First-Strand Buffer, 0.1 M DTT, 10 mM dNTP Mix, and DEPC-treated water. The SuperScript™ Direct cDNA Labeling System includes all components listed for the SuperScript™ cDNA Labeling Module plus purification columns, loading buffer, wash buffer, and amber collection tubes. Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.
Final Product Type cDNA (Labeled)
Label or Dye Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5
Includes Label or Dye No
Labeling Target cDNA
Labeling Method Direct Labeling
Sample Type RNA
Product Line SuperScript
Quantity 30 Reactions
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For Research Use Only. Not for use in diagnostic procedures.