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Invitrogen™ S1 Nuclease

Catalog No. 18001016
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20,000 U
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Catalog No. Quantity
18001016 20,000 U
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Catalog No. 18001016 Supplier Invitrogen™ Supplier No. 18001016
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Description

Includes

S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300mM sodium acetate (pH 4.6), 10mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3M NaCl.

Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides

Hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.

  • S1 Nuclease is stable at 65°C.
  • Applications: Nuclease mapping techniques, Removal of single-stranded regions from double-stranded DNA, Exo III-ordered sequencing
  • Source: Isolated from Aspergillus oryzae.
  • Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays
  • Unit Definition: One unit hydrolyzes 1μg of denatured DNA to acid-soluble material in 1 min. at 37°C
  • Unit Reaction Conditions: 30mM sodium acetate (pH 4.6), 50mM NaCl, 1mM zinc acetate, 0.5 mg/mL heat-denatured DNA, 5% (v/v) glycerol, and enzyme in 0.5mL for 10 min. at 37°C

Cloning, DNA and RNA Purification and Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Restriction Enzyme Cloning

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300 mM sodium acetate (pH 4.6), 10 mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3 M NaCl. Store at -20°C.
Shipping Condition Approved for shipment on Wet or Dry Ice
Quantity 20,000 U
Product Type Nuclease

Frequently Asked Questions (FAQs)

What are the activities and applications of Exonuclease III, Mung Bean Nuclease, and S1 Nuclease?

Exonuclease III catalyzes the removal of mononucleotides from a 3'-OH terminus of duplexed DNA. It requires a substrate of double-stranded DNA containing a blunt end or a 3' recessed end. Exonuclease III also works at nicks to generate gaps.

S1 nuclease is an endonuclease specific for single-stranded DNA or RNA and can be used to study nucleic acid hybridization, mapping RNA start sites and RNA splice sites. This enzyme is five times more active on DNA than RNA, and it will digest all nucleic acids if the enzyme is added to the reaction in excess.

Mung bean nuclease is an endonuclease similar in action to S1 nuclease. Unlike S1 nuclease, it is used to generate blunt ended DNA from ss overhangs.

For Research Use Only. Not for use in diagnostic procedures.