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Invitrogen™ S.N.A.P.™ Plasmid DNA MidiPrep Kit
The S.N.A.P.™ MidiPrep Kit is medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10–100 mL of overnight culture.
Supplier: Invitrogen™ K191001
Includes: S.N.A.P. MidiPrep filtering columns, S.N.A.P. MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, 4X final wash, resuspension buffer, precipitation salt, and RNase A
Description
The S.N.A.P.™ MidiPrep Kit is medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10–100 mL of overnight culture (Figure 1). The S.N.A.P. MidiPrep procedure takes just 40 minutes.
- Lyse cells and filter debris with the S.N.A.P. filtering column
- Apply flow-through to the S.N.A.P.™ DNA binding column
- Wash the column
- Elute pure, supercoiled plasmid DNA in water or TE buffer
Specifications
| Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| Spin Column | |
| Bacterial Culture | |
| Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription | |
| 100-200 μg (Medium-Scale) Plasmid DNA | |
| Plasmid DNA MidiPrep Kit | |
| Plasmid DNA |
| Kit | |
| Midi | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| S.N.A.P. | |
| 20 preps | |
| 40 min. |
For Research Use Only. Not for use in diagnostic procedures.