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Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit
Specifically designed to isolate high-quality viral nucleic acids from variety of RNA and DNA viruses
Manufacturer: Invitrogen™ LS12280050
Includes:
- 32mL Viral Lysis buffer (L22)
- 15mL 5X Wash Buffer (W5)
- 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
- 310μg lyophilized Carrier RNA
- 15.5mL Sterile, RNAase-free water
- 50 Viral Spin Columns with Collection Tubes
- 2 x 50 Wash Tubes (2.0mL)
- 50 Recovery Tubes (1.5mL)
Description
- PureLink Viral RNA/DNA Mini Kit provides rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants
- Designed to isolate high-quality viral nucleic acids from variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis
- Purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and genotyping
- Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
- Purifies viral RNA and DNA from ≤500μL cell-free samples within 45 min
- Ability to elute viral nucleic acids in low elution volumes of 10 to 50μL to allow sensitive downstream analysis
DNA & RNA Purification & Analysis, DNA Extraction, Genomic DNA Purification, RNA Extraction, Total RNA Isolation, Total RNA from Liquid Samples (e.g. Serum, Virus), Viral RNA/DNA Purification, gDNA from Virus Samples
Order Info
Shipping Condition: Room Temperature
Specifications
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting | |
Plasma, Cell-Free Fluids, Cerebrospinal Fluid, Serum, Liquid Samples (e.g. Serum), Viral Samples | |
The PureLink® Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions. Kit Contents: • 32 ml Viral Lysis buffer (L22) • 15 ml 5X Wash Buffer (W5) • 2 × 1.6 ml Proteinase K (20 mg/ml in storage buffer) • 310 µg lyophilized Carrier RNA • 15.5 ml Sterile, RNAase-free water • 50 Viral Spin Columns with Collection Tubes • 2 × 50 Wash Tubes (2.0 ml) • 50 Recovery Tubes (1.5 ml) Store all components except for Carrier RNA at room temperature. Carrier RNA should be stored at −20°C. |
|
Room Temperature |
Viral DNA⁄RNA | |
Silica Spin Column | |
Not High-throughput Compatible (Manual) | |
PureLink™ |
For Research Use Only. Not for use in diagnostic procedures.