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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit DFS Item

Contaminants are removed with Wash Buffer

$116.00 - $536.00

Specifications

For Use With (Application) Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
Isolation Technology Silica Spin Column
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Includes: Resuspension Buffer (R3)
RNase A (20 mg/mL)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
K210010
View Documents
Invitrogen™
LSK210010
50 preps Each for $116.00
 
K210011
View Documents
Invitrogen™
LSK210011
250 preps Each for $536.00
 
Description

Description

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit:
  • Fast—ready-to-use plasmid DNA typically in about 30 min
  • Flexible—formats work with vacuum and centrifugation platforms
Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 µg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
Silica Spin Column

For Research Use Only. Not for use in diagnostic procedures.