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  5. Invitrogen™ PureLink™ Quick Gel Extraction Kit

Invitrogen™ PureLink™ Quick Gel Extraction Kit
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Catalog No. K210012
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Description

Includes

Gel Solubilization Buffer (L3)
Wash Buffer (W1)
Elution Buffer (E5); (10 mM Tris-HCl, pH 8.5)
Quick Gel Extraction Columns
Wash Tubes
Recovery Tubes

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages.

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ∼30 minutes using PureLink™ silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum.

Advantages of using PureLink™ Quick Gel Extraction Kit:

  • Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points
  • Complete the procedure in ∼30 minutes
  • Easily purify DNA fragments from 40 bp to 10 kb from gels
  • Obtain high recovery of DNA fragments
  • Bind and purify up to 15 μg DNA with one column
  • Purify DNA fragments that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling

Note: The PureLink™ Quick Gel Extraction Kit is not designed to purify supercoiled plasmid DNA or genomic DNA from agarose gels. Only linear DNA fragments may be purified from gels using this kit.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage PureLink Elution Tubes
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Isolation Technology Silica Spin Column
Sample Type Agarose Gels, Gel Samples
Elution Volume 50 μL
Final Product Type DNA
For Use With (Application) PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Label or Dye Ethidium Bromide, SYBR Safe
Quantity 50 Preps
Shipping Condition Room Temperature
Test Time 30 min.
Yield 15 μg (Binding capacity)
Starting Material Amount ≤400 mg
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For Research Use Only. Not for use in diagnostic procedures.