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Invitrogen™ PureLink™ Genomic DNA Mini Kit DFS Item

No detectable contamination from carryover of protein or guanidine

$68.50 - $910.00

Specifications

For Use With (Application) Genotyping, PCR, Real-Time PCR, Sequencing, Southern Blotting
Sample Size 100 μl - 1mL Blood Samples ( Medium-Scale)
Storage Requirements Store all components at room temperature.
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



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Specifications

Description

PureLink Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

  • Kit flexibility—one kit works with a variety of sample types and sizes
  • Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
  • Improved design—optimized spin-column design and buffer formulation for better yield and purity
  • Multiple sample sources, one kit—Optimized components and protocols

  • PureLink Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use
  • Columns are newly configured to bind and release DNA more efficiently
  • Each sample source uses specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination
  • Chaotropic salt buffer promotes stable binding of DNA to the column resin and powerful wash buffers remove all traces of protein and salt
  • DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage
Higher yields and purity
  • Expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells
  • Enhanced PureLink lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∽1.9 for both A260/A280 and A260/A230

DNA & RNA Purification & Analysis, DNA Extraction, Epigenetic Sequencing, Genomic DNA Purification, Methylation Analysis by Sequencing, SOLiD™ Next-Generation Sequencing, SOLiD™ Next-Generation Sequencing Sample Purification, Sample & Library Preparation for SOLiD™ Next-Generation Sequencing, Sequencing, gDNA from Animal Tissues & Cells, gDNA from Bacteria, gDNA from Blood (Medium-Scale, 100μL-1mL)

Order Info

Shipping Condition: Room Temperature

Description & Specifications

Specifications

Genotyping, PCR, Real-Time PCR, Sequencing, Southern Blotting
100 μl - 1mL Blood Samples ( Medium-Scale)
Store all components at room temperature.