Invitrogen™ Proteinase K, recombinant

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

• Over a wide pH range–optimal activity between 6.5 and 9.5
• Under denaturing conditions–e.g., in the presence of SDS or urea
• In the presence of metal chelating agents–e.g., EDTA
• At comparatively high temperatures–optimum digestion temperature is 65°C
• Protein Filter: P

Applications

Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing

Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition

One mAnson unit is described as that amount of enzyme that liberates 1μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.