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Invitrogen™ Platinum™ ThermoScript™ Quantitative RT-PCR One-Step System DFS Item

Used for real-time quantitation of RNA molecules from total or poly(A)+ RNA in single step

$1,121.40 - $4,557.00

Specifications

Sample Type RNA
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Includes:  ThermoScript RT/Platinum Taq mix, and 2X ThermoScript reaction mix (buffer containing 0.4mM of each dNTP, 6mM MgSO4 ), vial of 50mM MgSO4

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

11731015

DFS Item
 
life technologies
11731015
100 reactions Each for $1,121.40

11731023

DFS Item
 
life technologies
11731023
500 reactions Each for $4,557.00
Description & Specifications

Specifications

Sample Type RNA

ThermoScript RT, a cloned avian reverse transcriptase, has been engineered for reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are enhanced through use of Platinum™ Taq DNA polymerase. The system can be used to quantitate the amount of specific mRNA from small numbers of cells and is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR.

  • Provides reduced RNase H activity for greater first-strand cDNA yield
  • Takes place sequentially in single tube, under conditions optimal for both ThermoScript RT and Platinum Taq DNA polymerase
  • Reduces reaction-to-reaction variability for improved precision in quantitative RT-PCR
  • Detects as little as 10 molecules of RNA template<br>•Allows precise quantification of mRNA(s) from 0.1pg to 1μg of total RNA

Gene Expression Analysis and Genotyping, One-Step qRT-PCR, PCR & Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

Shipping Condition: Dry ice