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Invitrogen™ Platinum™ SuperScript™ CellsDirect™ One-Step qRT-PCR Kit with ROX DFS Item

RNA purification step not needed, saves time and money

$877.80 - $3,843.00

Specifications

Sample Type Cells
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

11754100

DFS Item
 
life technologies
11754100
10mL Resuspension Buffer; 1mL Lysis Enhancer; 500μL DNase I, Amplification Grade (1U/μL); 160μL 10X DNase I Buffer; 400μL 25mM EDTA; 100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X Reaction Mix with ROX; 1mL 50mM MgSO4; 2mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL) 100 reactions Each for $877.80

11754500

DFS Item
 
life technologies
11754500
10mL Resuspension Buffer; 1mL Lysis Enhancer; 2 x 1.25mL DNase I, Amplification Grade (1U/μL); 800μL 10X DNase I Buffer; 2 x 1mL 25mM EDTA; 500μL SuperScript III RT/Platinum Taq Mix; 12.5mL 2X Reaction Mix with ROX; 1mL 50mM MgSO4; 12.5mL DEPC-treated water<; 10μL HeLa Total RNA (10ng/μL) 500 reactions Each for $3,843.00
Description & Specifications

Specifications

Sample Type Cells

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Streamline Your Workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for Your qRT-PCR Application CellsDirect technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™) or LUX™ fluorogenic primers.

  • Efficiency: Offers significant time and cost savings for increased throughput
  • Convenience: Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
  • Sensitivity: No separate RNA isolation step increases sensitivity by minimizing sample loss
  • Broad Detection Range: Detect targets from samples ranging from a single cell to 10,000 cells
  • Versatility: Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
  • For use with ABI 7000, 7300, 7700, and 7900(HT) instruments

This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit containing a separate tube of ROX Reference Dye, is also available.

Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples

Shipping Condition: Dry ice