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Invitrogen™ Platinum™ PCR SuperMix High Fidelity DFS Item

Built-in hot start

$340.20 - $340.20

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 6X
Gel Compatibility Gel Compatibility: Low
Storage Requirements Store at -20°C in a non-frost-free freezer.
View More Specs
Includes:  Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.
Requires:  Template and primers

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12532016

DFS Item
 
life technologies
12532016
100 reactions Each for $340.20

12532024

DFS Item
 
life technologies
12532024
5000 reactions CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description & Specifications

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 6X
Gel Compatibility Gel Compatibility: Low
Storage Requirements Store at -20°C in a non-frost-free freezer.

PlatinumPCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half.

  • Achieve greater than six-times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 15 kb
  • Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
  • Minimize PCR optimization

Using PlatinumPCR SuperMix, High Fidelity
PlatinumPCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic “hot-start” PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

High Fidelity PCR, Hot Start PCR, Long Fragment PCR, PCR, PCR and Real-Time PCR

Approved for shipment on Wet or Dry Ice