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  7. Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Catalog No. 12532024
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12532024 5000 Reactions
12532016 100 Reactions
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Catalog No. 12532024 Supplier Invitrogen™ Supplier No. 12532024

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.

Requires

Template and primers

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.

High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.

Benefits of Platinum PCR SuperMix, High Fidelity

  • Six-times higher fidelity than Taq DNA polymerase
  • Amplifies fragments up to 15 kb
  • Minimizes PCR optimization
  • Room-temperature reaction assembly

Applications

  • High-fidelity PCR
  • Cloning
  • Mutagenesis

Notes

  • Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
  • Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Concentration 1.1X
Content And Storage Platinum PCR SuperMix High Fidelity (4 x 56.25 mL)

Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance Low
Polymerase Platinum Taq DNA Polymerase High Fidelity
Reaction Speed Standard
Quantity 5,000 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 6X
Hot Start Built-In Hot Start
No. of Reactions 5000 Reactions
Overhang Mixed
Reaction Format SuperMix or Master Mix
Size (Final Product) 15 kb or less
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Frequently Asked Questions (FAQs)

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use MgCl2 instead of MgSO4 with Platinum Taq High Fidelity enzyme?

We strongly suggest using MgSO4. While MgCl2 may work in some cases, MgSO4 usually produces more robust and reproducible products, as sulfate is the best anion found for the Platinum Taq High Fidelity enzyme.

If I do PCR using the Platinum PCR SuperMix High Fidelity, can I use DNA-RNA chimeric oligos such as 5'-AGGCTTggau (lower case letters for RNA bases)?

PCR cannot be performed with an RNA oligo. RNA is not heat stable so the RNA part of the oligo will be degraded. In the presence of Mg++ (PCR buffer contains usually 1-2 mM Mg++) the rate of degradation is much faster.

For Research Use Only. Not for use in diagnostic procedures.