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Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity 
Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
Supplier: Invitrogen™ 11304011
Description
Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum™ automatic 'hot-start' technology. Features of Platinum™ Taq DNA Polymerase, High Fidelity:
• Fidelity—greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size—amplification of fragments up to 15 kb (see figure)
• Convenience—room temperature reaction assembly
• Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit definition
One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Specifications
| • Platinum Taq DNA Polymerase High Fidelity (1 x 20 μL at 5 U/μL) • 10X High Fidelity Buffer (1 x 1.25 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -10°C to -30°C. |
|
| Low | |
| Standard | |
| Dry Ice | |
| 6X | |
| 100 Reactions | |
| Separate Components |
| Primer-probe | |
| Platinum Taq DNA Polymerase High Fidelity | |
| DNA Polymerase High Fidelity | |
| Hot-start PCR, High-fidelity PCR | |
| Built-In Hot Start | |
| Mixed | |
| 20 kb or less |
For Research Use Only. Not for use in diagnostic procedures.