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Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase 
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.
$926.00 - $6530.00
Specifications
| GC-Rich PCR Performance | Low |
|---|---|
| Polymerase | Tsp Genotyping Polymerase |
| Reaction Speed | Standard |
| Shipping Condition | Wet or Dry Ice |
| For Use With (Application) | Hot-start PCR |
Includes: Platinum GenoType TspDNA Polymerase is supplied with 10X PCR Buffer (200mM Tris-HCl (pH 8.4), 500mM KCl), and a vial of 50mM MgCl2.
Description
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic hot-start method that improves PCR specificity and allows for room temperature reaction assembly.
Platinum GenoType Tsp DNA Polymerase features
- Minimal non-templated nucleotide addition to PCR products
- Lacks both 5' and 3' exonuclease activity
- Amplifies fragments up to 500 bp
- Room temperature reaction assembly
Application
Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.
Unit definition
One unit of Platinum GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C under optimized reaction conditions.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| Low | |
| Standard | |
| Hot-start PCR | |
| Built-In Hot Start | |
| Separate Components |
| Tsp Genotyping Polymerase | |
| Wet or Dry Ice | |
| 1X | |
| Blunt | |
| 500 bp or less |
For Research Use Only. Not for use in diagnostic procedures.