PlatinumGenoType TspDNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic “hot-start” method that improves PCR specificity and allows for room temperature reaction assembly.
Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.
One unit of PlatinumGenoType TspDNA Polymerase has been functionally determine to be equivalent to one unit of TaqDNA Polymerase in amplification of dinucleotide repeats using standard TaqDNA polymerase reaction conditions. One TspDNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C under optimized reaction conditions.
Hot Start PCR, PCR, PCR and Real-Time PCR, PCR Genotyping
Approved for shipment on Wet or Dry Ice
|Fidelity (vs. Taq): 1X|
|Gel Compatibility: Low|
|Store at -20°C. Guaranteed stable for 6 months when properly stored.|