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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

$1,106.00 - $2,270.00

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Vector Price Quantity  
Catalog Number Mfr. No. Quantity Vector Price Quantity  
K480001 Invitrogen™
LSK480001
20 reactions TOPO-TA Vectors, pcDNA Each for $1,294.00
K480040 Invitrogen™
LSK480040
40 reactions TOPO-TA Vectors, pcDNA Each for $2,270.00
K480001SC Invitrogen™
LSK480001SC
20 reactions pcDNA Each for $1,106.00
Specifications

Description

  • No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
  • Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
  • pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
  • C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody • Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
  • Vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Description & Specifications

Specifications

Constitutive
CMV
His Tag (6X), V5 Epitope Tag
Transfection
Constitutive Expression
Geneticin™ (G-418)