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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

$1,075.20 - $2,220.75

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Vector Price Quantity    

K480001

DFS Item
 
invitrogen™
K480001
20 reactions TOPO-TA Vectors, pcDNA Each for $1,247.40

K480040

DFS Item
 
invitrogen™
K480040
40 reactions TOPO-TA Vectors, pcDNA Each for $2,220.75

K480001SC

DFS Item
 
invitrogen™
K480001SC
20 reactions pcDNA Each for $1,075.20
Description & Specifications

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
Selection Agent (Eukaryotic) Geneticin™ (G-418)

  • No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
  • Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
  • pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
  • C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody • Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
  • Vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Shipping Condition: Dry Ice