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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit
Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector
$2,340.00 - $4,030.00
Specifications
| Constitutive or Inducible System | Constitutive |
|---|---|
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | TOPO TA Expression Kit |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Catalog Number | Mfr. No. | Vector | Quantity | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Vector | Quantity | Price | Quantity & Availability | ||||
K480001
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Invitrogen™
K480001 |
TOPO-TA Vectors, pcDNA | 20 Reactions |
Each for $2,340.00
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K480040
|
Invitrogen™
K480040 |
TOPO-TA Vectors, pcDNA | 40 Reactions |
Each for $4,030.00
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Description
- No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
- Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
- pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
- C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
- C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
- Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
- Vector features strong CMV promoter for high-level, constitutive expression
- C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| TOPO-TA | |
| TOPO, pcDNA |
| Transfection | |
| TOPO TA Expression Kit | |
| His Tag (6x), V5 Epitope Tag | |
| TOPO-TA Vectors, pcDNA | |
| Constitutive Expression |
For Research Use Only. Not for use in diagnostic procedures.