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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Supplier: Invitrogen™ K480040

Catalog No. K480040

Quantity

Description

No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
Vector features strong CMV promoter for high-level, constitutive expression
C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Shipping Condition: Dry Ice

Specifications

Chemical Identifiers

Constitutive or Inducible System	Constitutive
Promoter	CMV
Selection Agent (Eukaryotic)	Geneticin™ (G-418)
Cloning Method	TOPO-TA
Product Line	TOPO, pcDNA

Constitutive or Inducible System	Constitutive
Promoter	CMV
Selection Agent (Eukaryotic)	Geneticin™ (G-418)
Protein Tag	His Tag (6x), V5 Epitope Tag
Quantity	40 Reactions
Product Line	TOPO, pcDNA

Delivery Type	Transfection
Product Type	TOPO TA Expression Kit
Content And Storage	2 × Box 1 (store at -20°C) • 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl) • 100 μl 10X PCR Buffer • 10 μl dNTP Mix (50 mM) • 50 μl Salt Solution • 1 ml Sterile Water • 10 μl Control PCR Template (50 ng/μl) • 10 μl Control PCR Primers (100 ng/μl each) • 20 μl T7 Sequencing Primer (100 ng/μl) • 20 μl BGH Reverse Sequencing Primer (100 ng/μl) • 10 μl Expression Control Plasmid (500 ng/μl) 2 × Box 2 (store at -80°C) • 21 × 50 μl TOP10 E. coli cells • 50 μl pUC19 Control DNA (10 pg/μl) • 6 ml S.O.C. Medium
Cloning Method	TOPO-TA
Vector	TOPO-TA Vectors, pcDNA
For Use With (Application)	Constitutive Expression

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For Research Use Only. Not for use in diagnostic procedures.