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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression
$2,395.00 - $4,060.00
Spécifications
| Constitutive or Inducible System | Constitutive |
|---|---|
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | TOPO Expression Kit |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
Comprend: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA
| Numéro de catalogue | Numéro du manufacturier. | Quantity | Prix | Quantity & Availability | |||||
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| Numéro de catalogue | Numéro du manufacturier. | Quantity | Prix | Quantity & Availability | |||||
K490001
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Invitrogen™
K490001 |
20 Reactions |
chaque for $2,395.00
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Connectez-vous ou enregistrez-vous pour vérifier votre prix et la disponibilité.
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K490040
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Invitrogen™
K490040 |
40 Reactions |
chaque for $4,060.00
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Connectez-vous ou enregistrez-vous pour vérifier votre prix et la disponibilité.
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Description
- Proofreading enzyme is used: fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
- CMV promoter: high expression levels
- C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
- 6xHis tag for rapid protein purification on nickel-chelating resin
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Spécifications
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| Directional TOPO | |
| TOPO, pcDNA |
| Transfection | |
| TOPO Expression Kit | |
| His Tag (6x), V5 Epitope Tag | |
| Directional TOPO Vectors, pcDNA | |
| Constitutive Expression |
For Research Use Only. Not for use in diagnostic procedures.