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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit DFS Item

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

$1,326.00 - $2,295.00


Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
View More Specs


For Research Use Only. Not for use in diagnostic procedures.

Includes: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
K490001 Invitrogen™
20 reactions Each for $1,326.00
K490040 Invitrogen™
40 reactions Each for $2,295.00


  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Description & Specifications


His Tag (6X), V5 Epitope Tag
Directional TOPO Vectors, pcDNA
Constitutive Expression
Geneticin™ (G-418)