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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit DFS Item

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

$1,276.80 - $2,247.00

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
View More Specs
Includes:  Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

K490001

DFS Item
 
invitrogen™
K490001
20 reactions Each for $1,276.80

K490040

DFS Item
 
invitrogen™
K490040
40 reactions Each for $2,247.00
Description & Specifications

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Protein Tag His Tag (6X), V5 Epitope Tag
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Vector Directional TOPO Vectors, pcDNA

  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Shipping Condition: Dry Ice