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Invitrogen™ Taq DNA Polymerase, recombinant

Catalog No. 10342020
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10342020 500 Reactions
10342053 100 Reactions
10342046 1500 Reactions
10342178 5000 Reactions
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Catalog No. 10342020 Supplier Invitrogen™ Supplier No. 10342020
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Description

Includes

Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

Concentration 5 U/μL
Content And Storage • Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR buffer, 2.5 mL
• 50 mM MgCl2, 1 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.
Format Stand-alone enzyme
GC-Rich PCR Performance Low
PCR Method qPCR, Standard PCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 500 Units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 500 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.