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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli 
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Supplier: Invitrogen™ K250020
Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.
Description
- Contains minimal MCS, so sequence more of insert and less of vector
- Patented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
- att sites for rapid recombination into a variety of Gateway destination vectors
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Sequencing primer sites within 55 bp of insertion site
- Spectinomycin resistance gene for robust selection in E. coli
- EcoRI sites flanking the PCR product insertion site for easy excision of inserts
Cloning,Gateway Cloning,PCR Cloning
Specifications
| TOPO™ with Gateway™ | |
| 20 reactions | |
| TA Cloning Kit | |
| pCR |
| The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| One Shot | |
| 20 Reactions |
For Research Use Only. Not for use in diagnostic procedures.