missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. K252002

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit

Catalog No. K252002
Encompass
Change view
Click to view available options
Quantity:
20 Preps
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K252002 20 Preps
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K252002 Supplier Invitrogen™ Supplier No. K252002
Only null left

Description

Includes

pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes.

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

  • pCR™8 TOPO TA vector contains minimal MCS, so sequence more of insert and less of vector
  • Patented att sites make pCR™8 vector is simplest way to create an entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
  • pCR™8/GW/TOPO TA Cloning Kit features fast growing E. coli that shortens cloning time
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Sequencing primer sites within 55 bp of insertion site
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Specifications

Cloning Method TOPO™ with Gateway™
Content And Storage pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
No. of Reactions 20 preps
Product Line One Shot
Product Type TA Cloning Kit
Quantity 20 Preps
Vector pCR

For Research Use Only. Not for use in diagnostic procedures.