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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

Catalog No. K252020
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20 Reactions
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K252020 20 Reactions
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Catalog No. K252020 Supplier Invitrogen™ Supplier No. K252020
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Description

Includes

TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

  • Fast, 5-minute TOPO TA Cloning delivers 95% recombinants
  • 3'-T overhangs simplify cloning of Taq-amplified PCR products
  • Fast growing MachI cells shorten cloning time
  • Sequencing primer sites located close to cloning insertion site so sequences more insert, less vector first time
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Specifications

Cloning Method TOPO™ with Gateway™
Content And Storage The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
No. of Reactions 20 reactions
Product Line One Shot
Product Type TA Cloning Kit
Quantity 20 Reactions
Vector pCR

For Research Use Only. Not for use in diagnostic procedures.