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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli 
Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications
Supplier: Invitrogen™ K252020
Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.
Description
- Fast, 5-minute TOPO TA Cloning delivers 95% recombinants
- 3'-T overhangs simplify cloning of Taq-amplified PCR products
- Fast growing MachI cells shorten cloning time
- Sequencing primer sites located close to cloning insertion site so sequences more insert, less vector first time
- Spectinomycin resistance gene for robust selection in E. coli
- EcoR I sites flanking PCR product insertion site for easy excision of inserts
Cloning,Gateway Cloning,PCR Cloning
Specifications
| TOPO™ with Gateway™ | |
| 20 reactions | |
| TA Cloning Kit | |
| pCR |
| The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored | |
| One Shot™ | |
| 20 Reactions |
For Research Use Only. Not for use in diagnostic procedures.