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  5. Invitrogen™ ChargeSwitch™-Pro Plasmid Miniprep Kit

Invitrogen™ ChargeSwitch™-Pro Plasmid Miniprep Kit

Catalog No. CS30010
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CS30010 10 Preps
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Catalog No. CS30010 Supplier Invitrogen™ Supplier No. CS30010
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Description

Includes

Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer

The ChargeSwitch™-Pro Plasmid Miniprep Kit generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications.

The ChargeSwitch™-Pro Plasmid Miniprep Kit generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications.

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• Ready-to-use, complete kit for a fast, hassle-free start
• Ideal for purifying plasmid DNA from 1-5 mL overnight bacterial cultures, yielding up to 20 μg of plasmid DNA

Order Info

Shipping Condition: Room Temperature

Specifications

Column Type Spin Column
Content And Storage Store at room temperature.
Format Kit
Isolation Technology ChargeSwitch Chemistry
Scale Mini
Sample Type Bacterial Culture
Final Product Type Plasmid DNA
For Use With (Application) PCR, Southern Blotting, Sequencing, Cloning
High-throughput Compatibility Not High-throughput Compatible (Manual)
No. of Reactions 10 Preps
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Product Line ChargeSwitch
Product Type Plasmid MiniPrep Kit
Quantity 10 Preps
Shipping Condition Room Temperature
Target Plasmid DNA
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Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.