Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

ChargeSwitch coated beads are inert and will not affect PCR. We have found that volumes of as much as 10 µL of beads have no deleterious effects on regular PCR when spiked in. Excessive quantities greater than 10 µL of beads will start to inhibit PCR. Some specific applications may be affected by beads, including real-time reactions or MALDI-TOF. In these applications, repeat the final elution binding as described in the protocol.

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.