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Invitrogen™ ChargeSwitch™ Plasmid Yeast Mini Kit 
The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media.
Supplier: Invitrogen™ CS10203
Includes:
- ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
- RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
- ChargeSwitch Resuspension Buffer (R4)
- ChargeSwitch Lysis Buffer (L9)
- ChargeSwitch Precipitation Buffer (N5)
- ChargeSwitch Wash Buffer (W11)
- ChargeSwitch Wash Buffer (W12)
- ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Description
The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media. Data generated from transformation and PCR experiments using ChargeSwitch purified DNA shows improved performance versus DNA purified by other methods.
- Sample size 1 mL S. cerevisiae culture
- Typical yield up to 25 ng
- Increased transformation efficiency
- Reagents that avoid PCR inhibition
- Purifies DNA with improved downstream performance
- Includes all necessary reagents for number of preps Listed and requires use of magnetic accessory
Order Info
Shipping Condition: Room Temperature
Specifications
All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
|
| ChargeSwitch Chemistry | |
| Yeast | |
| PCR, Cloning, Southern Blotting, Sequencing, Transformation | |
| Not High-throughput Compatible (Manual) | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid Yeast Mini Kit | |
| Room Temperature |
| Kit | |
| Mini | |
| Plasmid DNA | |
| Less hazardous, Fewer resources used and less waste | |
| 50 Preps | |
| ChargeSwitch | |
| 50 Preps | |
| Plasmid DNA |
For Research Use Only. Not for use in diagnostic procedures.