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Invitrogen™ ChargeSwitch™ Plasmid Yeast Mini Kit
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Catalog No. CS10203
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50 Preps
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CS10203 50 Preps
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Catalog No. CS10203 Supplier Invitrogen™ Supplier No. CS10203
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Description

Includes

  • ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch Resuspension Buffer (R4)
  • ChargeSwitch Lysis Buffer (L9)
  • ChargeSwitch Precipitation Buffer (N5)
  • ChargeSwitch Wash Buffer (W11)
  • ChargeSwitch Wash Buffer (W12)
  • ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media.

The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media. Data generated from transformation and PCR experiments using ChargeSwitch purified DNA shows improved performance versus DNA purified by other methods.

  • Sample size 1 mL S. cerevisiae culture
  • Typical yield up to 25 ng
  • Increased transformation efficiency
  • Reagents that avoid PCR inhibition
  • Purifies DNA with improved downstream performance
  • Includes all necessary reagents for number of preps Listed and requires use of magnetic accessory

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage

All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Resuspension Buffer (R4)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format Kit
Isolation Technology ChargeSwitch Chemistry
Scale Mini
Sample Type Yeast
Final Product Type Plasmid DNA
For Use With (Application) PCR, Cloning, Southern Blotting, Sequencing, Transformation
High-throughput Compatibility Not High-throughput Compatible (Manual)
No. of Reactions 50 Preps
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Product Line ChargeSwitch
Product Type Plasmid Yeast Mini Kit
Quantity 50 Preps
Shipping Condition Room Temperature
Target Plasmid DNA
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Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.