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  5. Invitrogen™ ChargeSwitch™ gDNA Plant Kit

Invitrogen™ ChargeSwitch™ gDNA Plant Kit
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Catalog No. CS1800010
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No. of Reactions:
96 Preps
960 Preps
Sample Type:
Fungi, Plants
Leaf, Seeds
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Catalog No. No. of Reactions Sample Type
CS1800010 960 Preps Leaf, Seeds
CS18000 96 Preps Fungi, Plants
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Catalog No. CS1800010 Supplier Invitrogen™ Supplier No. CS1800010
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Description

Includes

ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch Lysis Buffer (L18)
ChargeSwitch Precipitation Buffer (N5)
ChargeSwitch Wash Buffer (W12)
ChargeSwitch Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)
RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
10% SDS in deionized water
10% Detergent (D1)

The ChargeSwitch™ gDNA Plant Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from fungal and plant samples, including leaves, tissues and seeds, in less than 15 minutes after lysate preparation.

The ChargeSwitch™ gDNA Plant Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from fungal and plant samples, including leaves, tissues and seeds, in less than 15 minutes after lysate preparation.

• Optimized for higher-purity DNA extraction
• Compatible with high-throughput protocols
• Ideal for a variety of downstream applications
• Less hazardous, less waste

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage

All components of the ChargeSwitch™ gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4°C. Store all other kit components at room temperature.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• ChargeSwitch™ Lysis Buffer (L18)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• 10% SDS in deionized water
• 10% Detergent (D1)
Format Kit
Isolation Technology ChargeSwitch Chemistry
Sample Type Leaf, Seeds
For Use With (Application) PCR, Southern Blotting, Sequencing, Cloning
High-throughput Compatibility High-throughput Compatible
No. of Reactions 960 Preps
Product Line ChargeSwitch
Product Type gDNA Plant Kit
Quantity 960 Preps
Shipping Condition Room Temperature
Target Genomic DNA
Test Time 15 min.
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Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.