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Invitrogen™ ChargeSwitch™ Direct gDNA Purification Kit, 96-well
Description
Includes
ChargeSwitch Plate Binding and lysis Buffer, ChargeSwitch Wash Buffers, ChargeSwitch Elution Buffer, ChargeSwitch Direct 96 gDNA Plates, and Plate Seals.
- ChargeSwitch Direct 96 gDNA Kit is designed for rapid and efficient isolation of gDNA from fresh or frozen samples
- Utilizes 96-well plates coated with ChargeSwitch nucleic acid-binding surface, whose ionic charge can be switched by changing pH of surrounding buffer
- At low pH, positive charge of ChargeSwitch surface binds negatively charged nucleic acids
- Proteins and other contaminants that do not bind are washed away, leaving pure DNA ready for downstream applications
- Successful yields from very small samples volumes up to 10 μL blood or 2 x 104cells
- Highly pure DNA isolated using gentle buffers—no guanidine or ethanol
- No sample transfer that can lead to error or contamination—direct amplification in the same well as DNA binding without elution
- Adaptable to your throughput needs—compatible with most automated Liquid handling platforms
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Format | Kit |
| Isolation Technology | ChargeSwitch Chemistry |
| Sample Type | Blood, Cells |
| For Use With (Application) | PCR, Southern Blotting, Sequencing, Cloning |
| High-throughput Compatibility | High-throughput Compatible |
| Prep Scale | < 100 μL Blood Samples (Small-scale) |
| Product Line | ChargeSwitch |
| Product Type | Direct gDNA Purification Kit |
| Quantity | 96 Preps |
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Frequently Asked Questions (FAQs)
We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.
The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.
Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.
For Research Use Only. Not for use in diagnostic procedures.