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Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit

Catalog No. V48820
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Catalog No. V48820 Supplier Invitrogen™ Supplier No. V48820
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Description

Includes

The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot™ Stbl3™ Chemically Competent E.Coli.

For lentiviral delivery and genomic integration of DNA coding for shRNA

The BLOCK-iT Lentiviral RNAi Zeo Gateway Vector Kit contains the pLenti4/BLOCK-iT-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and initiates target-specific RNAi.

  • Gateway Technology for efficient recombination of the RNAi cassette from the BLOCK-iT inducible pENTR™/H1/TO vector
  • All required components for efficient lentiviral packaging, delivery, and integration of the shRNA
  • Zeocin selection marker for fast selection of clonal cell lines containing the RNAi cassette

Lentiviral shRNA and miRNA, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Virus-Based RNAi

Specifications

Product Type RNAi Expression Vector Kit
Content And Storage The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot™ Stbl3™ Chemically Competent E.Coli. Store the pLenti4/BLOCK-iT™-DEST, pLenti6/TR vector, Blasticidin, ViraPower™ Lentiviral packaging mix, and the control vector at -20°C. Store the One Shot Stbl3™ Competent Cells 293 FT cell, and LR Clonase™ enzyme mix at -80°C. Store Lipofectamine™ 2000 at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method Gateway™
Constitutive or Inducible System Inducible
Delivery Type Lentiviral
RNAi Type shRNA
Selection Agent (Eukaryotic) Zeocin™
Vector pLenti
Promoter H1/TO
Format Kit
Product Line BLOCK-iT, Gateway
Quantity 20 Reactions
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For Research Use Only. Not for use in diagnostic procedures.