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Invitrogen™ Platinum™ SuperFi™ PCR Master Mix
Description
Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.
Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.
Features
- Exceptional >300X Taq fidelity
- High specificity and increased yields with Platinum hot-start technology
- Robust amplification of difficult targets of high GC content
- Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
- Amplification of samples with suboptimal purity
Applications
- High-fidelity PCR
- Cloning and sub-cloning
- Site-directed mutagenesis
- Amplification of GC-rich templates
- Template generation for sequencing
- High-throughput PCR
- Long PCR
Notes
- Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
- Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).
Specifications
Specifications
| Content And Storage | 5 boxes of Platinum SuperFi Master Mix, each containing: • 2X Platinum SuperFi PCR Master Mix (10 x 1.25 mL) • SuperFi GC Enhancer (4 x 1.25 mL) • Nuclease-free water (10 x 1.25 mL) Store at –5°C to –30°C. |
| GC-Rich PCR Performance | High |
| Polymerase | Platinum SuperFi DNA Polymerase |
| Reaction Speed | Fast |
| Quantity | 5 x 500 reactions |
| Shipping Condition | Wet or Dry Ice |
| For Use With (Application) | Hot-start PCR, High-fidelity PCR |
| Fidelity (vs. Taq) | 300X |
| Hot Start | Built-In Hot Start |
| No. of Reactions | 5 x 500 Reactions |
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Frequently Asked Questions (FAQs)
Platinum SuperFi DNA Polymerase cannot read dUTP-derivatives or dITP in DNA templates, so the use of these analogues is not recommended.Platinum SuperFi DNA Polymerase can incorporate 7-deaza-dGTP and radiolabeled dNTPs.
Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh and intact) and fresh primer solutions. Optimization steps to consider include decreasing denaturation and extension temperatures, lengthening extension times as recommended in the manual, and increasing template amounts.
Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).
All Platinum SuperFi product formats are supplied with 5X SuperFi GC Enhancer which is optimized to improve amplification of GC-rich targets (recommended for use with targets containing >65% GC content)
No. The colored buffer does not interfere with PCR performance and does not change enzyme features.
For Research Use Only. Not for use in diagnostic procedures.