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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit

Catalog No. 45500134
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Catalog No. 45500134 Supplier Cytiva Supplier No. 29108107
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Description

Includes

Single Cell GenomiPhi Reaction buffer 1 x 275µL, Single Cell GenomiPhi Enzyme mix 1 x 25µL, Single Cell GenomiPhi Amplification mix 1 x 25µL, Single Cell GenomiPhi DTT Solution 1 x 100µL, Single Cell GenomiPhi Lysis buffer 1 x 100µL and Single Cell GenomiPhi Neutralisation buffer 1 x 100µL

Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

  • For whole genome amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
  • Reaction time: 2 to 4 hours
  • Product average size: >10kb
Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


Specifications

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
Description Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
Final Product Type illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
For Use With (Application) Genomic DNA Amplification
Includes Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
No. of Reactions 25
Product Type illustra Single Cell GenomiPhi DNA Amplification Kit
Size >10kb
Test Time 2 to 4 hours