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Cytiva HiTrap™ IMAC Columns

Catalog No. 45000163
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Quantity:
5 x 1 mL
5 x 5 mL
Diameter (English) Inner:
0.27 in.
0.62 in.
Particle Size:
34 μm
90 μm
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Catalog No. Quantity Diameter (English) Inner Particle Size
45000163 5 x 1 mL 0.27 in. 34 μm
45000164 5 x 5 mL 0.62 in. 34 μm
45000167 5 x 1 mL 0.27 in. 90 μm
45000168 5 x 5 mL 0.62 in. 90 μm
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Catalog No. 45000163 Supplier Cytiva Supplier No. 17092003
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Includes

Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion

  • Conveniently charge with your metal of choice
  • Can be used with a syringe, peristaltic pump, or liquid chromatography system
  • Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
  • High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
  • Stable in the presence of all commonly used buffers
  • Available in 1mL and 5mL sizes

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.27 in.
Flow Rate <4 mL/min.
Diameter (Metric) Inner 7 mm
Kit Contents IMAC Sepharose
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system
Material Polypropylene
Max. Pressure 42 psi
pH Range 3 to 12
Particle Size 34 μm
Product Type HiTrap IMAC HP Column
Content And Storage 4°C to 30°C
Quantity 5 x 1 mL
For Use With (Application) For optimizing purification of His-tagged Proteins
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