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Cytiva HiTrap™ Chelating HP Columns

Catalog No. p-3676401
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1 x 5mL
5 x 1mL
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Catalog No. Quantity
45000061 1 x 5mL
45000060 5 x 1mL
45000062 5 x 5mL
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Catalog No. 45000061 Supplier Cytiva Supplier No. 17040901

Description

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Bed Volume 5mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16mm
Quantity 1 x 5mL
Height (English) 0.98 in.
Height (Metric) 25mm
Flow Rate <20 mL/min.
Material Polypropylene
Ligand Type Iminodiacetic Acid
Max. Pressure 3 bar
pH Range 3 to 13
Particle Size 34μm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Affinity Column
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
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