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  6. Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Catalog No. p-3676336
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Quantity:
1 mL
5 x 5 mL
60 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Catalog No. Quantity Flow Rate
45000335 5 x 5 mL <20 mL/min.
45000000 60 mL <4 mL/min.
45000507 1 mL <4 mL/min.
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Catalog No. 45000335

Supplier: Cytiva 17528601

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Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Bed Volume 5 mL
Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Flow Rate <20 mL/min.
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 45 to 165 μm
Quantity 5 x 5 mL
For Use With (Application) Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
Type Pre-packed Column
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