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  6. Cytiva HisTrap™ FF Columns: 16 x 25mm

Cytiva HisTrap™ FF Columns: 16 x 25mm

Catalog No. 45000336
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Quantity:
5 x 1 mL
Catalog No. Quantity
45000336 5 x 1 mL
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Catalog No. 45000336

Supplier: Cytiva 17531901

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Description

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

Specifications

Bed Volume 5
Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate <20 mL/min.
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 5 x 1 mL
Type Pre-packed Column
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