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Cytiva HisTrap™ excel Prepacked Columns

Catalog No. p-4513005
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Quantity:
1 mL
5 x 1 mL
5 x 5 mL
Diameter (Metric) Inner:
7 mm
16 mm
Flow Rate:
1 to 4 mL/min.
5 to 20 mL/min.
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Catalog No. Quantity Diameter (Metric) Inner Flow Rate
45003013 5 x 1 mL 7 mm 1 to 4 mL/min.
45003014 5 x 5 mL 16 mm 5 to 20 mL/min.
45000505 1 mL 7 mm 1 to 4 mL/min.
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Catalog No. 45003013 Supplier Cytiva Supplier No. 17371205
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Description

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Flow Rate 1 to 4 mL/min.
Material Polypropylene
pH Range 2 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 5 x 1 mL
Type Pre-packed Column
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