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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

$213.15 - $3,711.75

Specifications

Description HisPur Ni-NTA Superflow Agarose
Formulation Nickel-NTA (IMAC) on highly crosslinked 6% beaded agarose (superflow 6), slurried in 20% ethyl alcohol
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Products
Catalog Number Mfr. No. Price Quantity    

PI25214

 
thermo scientific™
25214
Each for $213.15

PI25215

 
thermo scientific™
25215
Each for $879.90

PI25216

 
thermo scientific™
25216
Each for $3,711.75
Description & Specifications

Specifications

Description HisPur Ni-NTA Superflow Agarose
Formulation Nickel-NTA (IMAC) on highly crosslinked 6% beaded agarose (superflow 6), slurried in 20% ethyl alcohol

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).


Additional Information and Online Ordering »

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins