HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.
High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour
High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates
Versatile – can be used to purify proteins under native and denaturing conditions
Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour
Compatible – maintains function in a wide variety of chemicals and pH conditions
Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse
Medium- to large-scale FPLC purification of polyhistidine tagged proteins