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Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

$163.39 - $4,508.33


Support Type Agarose
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Products ${productFamilyLength}
Catalog Number Mfr. No. Description Quantity Price Quantity  
Catalog Number Mfr. No. Description Quantity Price Quantity  
PI88221 Thermo Scientific™
HisPur Ni-NTA Resin 10mL Pack for $163.39
PI88222 Thermo Scientific™
HisPur Ni-NTA Resin 100mL Pack for $1,132.45
PI88223 Thermo Scientific™
HisPur Ni-NTA Resin 500mL Pack for $4,508.33
PI88224 Thermo Scientific™
HisPur Ni-NTA Spin Columns, 0.2mL 25 columns Pack of 25 Each for $298.12
PI88225 Thermo Scientific™
HisPur Ni-NTA Spin Columns, 1mL 5 columns Each for $186.08
PI88226 Thermo Scientific™
HisPur Ni-NTA Spin Columns, 3mL 5 columns Pack of 5 Each for $294.97
PI88227 Thermo Scientific™
HisPur Ni-NTA Spin Purification Kit, 0.2mL 25 column kit Pack of 25 Each for $500.88
PI88228 Thermo Scientific™
HisPur Ni-NTA Spin Purification Kit, 1mL 5 column kit Pack of 5 Each for $473.60
PI88229 Thermo Scientific™
HisPur Ni-NTA Spin Purification Kit, 3mL 5 column kit Pack of 5 Each for $504.03
PI88230 Thermo Scientific™
HisPur Ni-NTA Spin Plates 2 plates Each for $702.46
PI90098 Thermo Scientific™
HisPur Ni-NTA Chromatography Cartridges, 1mL 5 cartridges Pack of 5 Each for $192.85
PI90099 Thermo Scientific™
HisPur Ni-NTA Chromatography Cartridges, 5mL 5 cartridges Pack of 2 Each for $332.17


Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.


  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

  • Versatile – purify proteins using native or denaturing conditions

  • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

  • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

  • Cost-effective – reuse the same batch of resin at least five times

  • Easy to use – pre-formulated buffers available for kit formats

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin


Kits contain HisPur Ni-NTA Columns

Description & Specifications