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Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 1 mL

Numéro de catalogue. PI88228
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5 Column Kit
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PI88228 5 Column Kit
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Numéro de catalogue. PI88228 Fournisseur Thermo Scientific™ Code fournisseur 88228
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Description

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

  • Versatile – purify proteins using native or denaturing conditions

  • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

  • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

  • Cost-effective – reuse the same batch of resin at least five times

  • Easy to use – pre-formulated buffers available for kit formats

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

Spécifications

Capacity (Metric) 1 mL/Column
Description HisPur Ni-NTA Spin Purification Kit, 1mL
Content And Storage Sufficient For: Purifying up to 60 mg of His-tagged protein per column
• HisPur Ni-NTA Spin Columns, 1 mL, 5 columns
• Phosphate-buffered Saline (10X), 30 mL
• Imidazole (2M), 8 mL
• Column Plugs, 5 plugs

Store at 4°C.
Product Line HisPur
Quantity 5 Column Kit
Target His-Tagged Protein
Type Spin Column Kit
Stationary Phase Divalent Nickel, Metal Chelate
Sufficient For 60 mg of His-Tagged Protein per Column
Format Spin Column
Column Type Affinity, Agarose Resin
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For Research Use Only. Not for use in diagnostic procedures.