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  6. Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Catalog No. p-4589188
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Quantity:
10 mL
50 mL
Catalog No. Quantity
PI25228 10 mL
PI25229 50 mL
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Catalog No. PI25228

Supplier: Thermo Scientific™ 25228

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Description

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Additional Information and Online Ordering

HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps.

Highlights:

  • Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator

  • High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate

  • High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins

  • Compatible – maintains function after exposure to a wide variety of chemicals and pH values

  • Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Large scale FPLC purification of polyhistidine tagged proteins

Specifications

Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur
Quantity 10 mL
Target His-Tagged Protein
Type Agarose
Stationary Phase Metal Chelate, Divalent Cobalt
Format Bottle
Column Type Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.