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  6. Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Catalog No. 45002547
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Ligand Type:
Blue Sepharose
DEAE Sepharose
Ni Sepharose
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Catalog No. Ligand Type
45002547 Ni Sepharose
45002546 Blue Sepharose
45002548 DEAE Sepharose
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Catalog No. 45002547 Supplier Cytiva Supplier No. 28978244
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Description

Used for method optimization and parameter screening

  • Based on 6% highly cross-linked agarose with an average bead size of 90μm
  • Processed flow properties and high loading capacities
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90μm
  • Flow velocity: <600cm/hr or <450cm/hr
  • Material (Column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

Blue Sepharose

  • Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
  • Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
Ni Sepharose
  • Prepacked with Nickel Sepharose 6 Fast Flow
  • Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
DEAE Sepharose
  • Prepacked with DEAE Sepharose Fast Flow
  • Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

Specifications

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type HiScreen Ni Sepharose 6 FF column
Bed Volume 4.7 mL
Diameter (English) Inner 0.3 in.
Diameter (Metric) Inner 7.7 mm
Quantity 1 x 4.7mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate <450 cm/hr.
Material Polypropylene
Ligand Type Ni Sepharose
Max. Pressure 1.5 bar
pH Range 3 to 12
Particle Size 45 to 165μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Prepacked Fast Flow Column
For Use With (Application) Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy
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