missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva His SpinTrap™ Spin Columns

Cytiva His SpinTrap™ Spin Columns

Catalog No. 45001427
Change view
Click to view available options
Type:
Spin Column
Spin Column Kit
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Type
45001427 Spin Column
45002017 Spin Column Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. 45001427 Supplier Cytiva Supplier No. 28401353
Only null left

Description

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

Bed Volume 100 μL
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Quantity 1 Pack His SpinTrap Spin Columns (50 x 100 μL)
Type Spin Column
Sample Volume 600 μl