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Cytiva HiPrep™ IMAC FF 16/10 Column

Catalog No. 45002072
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Catalog No. 45002072 Supplier Cytiva Supplier No. 28936552

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

For optimizing purification of histidine-tagged proteins at laboratory scale

IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up. The medium consists of 90μm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled. This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein.

Specifications:
IMAC Sepharose 6 Fast Flow

  • Matrix: highly crosslinked agarose, 6%
  • Average particle size: 90μm
  • Binding capacity (protein/ion dependent): approx. 40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Compatibility: stable in all commonly used buffers
  • Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr. tested 2% SDS; 30 min. tested 30% 2-propanol
  • Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term)
  • Storage/Temperature: 20% ethanol; 4° to 30°C

HiPrep Prepacked Columns

  • Volume: 20mL
  • Dimensions: 1.6 x 10cm
  • Max. pressure during operation: 1.5bar (0.15MPa, 22psi)
  • Hardware pressure limit: 5bar (0.5MPa, 73psi)
  • Recommend flowrate (H2O at room temp.): 2 to 10mL/min.

    • Specifications

    Buffer 0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
    Product Type HiTrap IMAC Sepharose 6 FF column
    Bed Volume 20mL
    Diameter (English) Inner 0.62 in.
    Diameter (Metric) Inner 16mm
    Quantity 1 x 20mL
    Height (English) 3.93 in.
    Height (Metric) 100mm
    Flow Rate <10mL/min.
    Material Polypropylene
    Max. Pressure 1.5 bar
    pH Range 3 to 12
    Particle Size 45 to 165μm
    Support Type 6% Cross-linked Agarose
    Content And Storage 4° to 30°C
    Column Type Ion Exchange Column
    For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
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