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Cytiva GSTrap™ HP Columns

Catalog No. 45000332
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1 x 5 mL
5 x 1 mL
5 x 5 mL
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45000332 5 x 1 mL
45000334 5 x 5 mL
45000333 1 x 5 mL
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Catalog No. 45000332 Supplier Cytiva Supplier No. 17528101
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Description

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

  • One-step purification of GST-tagged proteins and other glutathione S-transferases or glutathione-dependent proteins
  • Glutathione coupled to Sepharose™ High Performance media
  • Column material: polypropylene
  • May be used with syringe, peristaltic pump or chromatography system
  • Purification process preserves antigenicity and function
  • Available as 1mL and 5mL columns
Media
  • Average particle size: 34μm
  • Ligand: Glutathione
  • Matrix: Highly cross-linked 6% agarose
  • Binding capacity: >7mg/mL
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.27 in.
Diameter (Metric) Inner 7 mm
Description GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Flow Rate <4 mL/min.
Material Polypropylene
pH Range 3 to 12
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 34 μm
Quantity 5 x 1 mL
For Use With (Application) Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Type Pre-packed Column
Ligand Type Glutathione
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