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  6. Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Catalog No. p-3676593
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45000286 100 mL
45000285 25 mL
45000287 500 mL
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Catalog No. 45000286 Supplier Cytiva Supplier No. 17513202
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Description

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

  • Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
  • Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
  • Binding capacity and high flow properties allow scale-up
  • Mild elution conditions preserve protein antigenicity and function
  • Stable in all commonly used aqueous buffers
  • pH stability: 3 to 12

Specifications

Binding Capacity >10 mg/mL
Description Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Flow Rate <450 cm/hr.
Kit Contents 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Matrix Highly cross-linked 4% agarose
pH Range 3 to 12
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Particle Size 90 μm
Quantity 100 mL
Type Chromatography Media
Ligand Type Glutathione and 10-carbon linker arm
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